Phenotype Image Detail

Image

Caption

Axonal degeneration and neuron loss in Ei24^tm1Hzha/Ei24^tm1Hzha Tg(Nes-cre)1Kln/0 (Nes Ei24^f/f) mice. S1(D) Compared to control (Nes Ei24^f/+) mice, the alveus of the hippocampus of mutant mice shows disarranged nerve fibers and contains vacuolated cells. Bar, 20 um. (E) Axon fibers, stained by anti-Beta-tubulin III antibody, are irregularly arranged in the alveus of the hippocampus of mutant mice. Bar, 10 um. (F) H&E staining reveals thepresence of eosinophilic spheroids (arrow) in the cortex in mutant mice at 4 months of age. Bar, 20 um. (G) Anti-MBP staining reveals that myelin in the cingulate cortex is discontinuous in mutant mice at 4 months of age. Bar,100 um. (H) H&E staining of the DCN indicates that eosinophilic spheroids are absent from control mice but accumulate in mutant mice (arrows). Bar, 20 um. (I-J) Anti-calbindin and anti-MBP costaining reveals that compared to controls (I), mutant mice exhibit dilated calbindin-positive bulbs (J, arrows) in the DCN region and some of the bulbs are enwrapped by MBP-labeled myelin, indicating axon degeneration. Bar, 10 um. (K-N) EM pictures of DCN in control and mutant mice at 4 months of age. (K), Myelinated axons are regular in structure, shape and size in the DCN region of control animals. (L-N), Degenerated axons in mutant mice. (L), The myelin sheath is irregularly shaped, and the myelin lamellae are undulated and split with disorganized structures (arrows) and decreased density in certain regions (arrowhead). (M), The arrow indicates a degenerated axon with a wide area formed between split lamellae. The arrowhead shows a degenerated axon. (N), An axon showing pronounced degeneration. Bar, 1 um (K-N).

Copyright

This image is from Zhao YG, J Biol Chem 2012 Dec 7;287(50):42053-63 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:193420

Associated Alleles

Symbol	Name
Ei24^tm1Hzha	etoposide induced 2.4 mRNA; targeted mutation 1, Hong Zhang
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein

Associated Genotypes

Allelic Composition	Genetic Background
Ei24^tm1Hzha/Ei24^tm1Hzha Tg(Nes-cre)1Kln/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL