Phenotype Image Detail

Image

Caption

A block in ductal-side branching and alveologenesis in the mammary gland of hormone-treated Pgr^tm4.2Lyd/Pgr^tm4.2Lyd (PR^d/d) and Pgr^tm1Bwo/Pgr^tm1Bwo (PRKO) mice. (a) Whole-mount of a mammary gland obtained from a wild-type (WT) mouse previously treated with E2P4 for 3 weeks; (b) higher magnification image of a region of this tissue. Note the widespread presence of extensive ductal side-branching and alveologenesis. (c-f) show low and high-magnification images of mammary tissue derivedfrom the similarly treated Pgr^tm1Bwo/Pgr^tm1Bwo (PRKO) and Pgr^tm4.2Lyd/Pgr^tm4.2Lyd (PR^d/d) mouse, respectively. Note the absence of ductal side branches and alveolar bodies in the PRKO and PR^d/d mammary glands. (g, h) show numerous luminal epithelial cells scoring positive (arrowhead) in the alveolar and ductal compartment of the E2P4-treated WT mammary gland respectively. (i, j) show a single-cell registering positive for BrdU incorporation (arrowhead) in the ductal compartment of the similarly treated PRKO and PR^d/d mammary gland, respectively. (k) A histogram displaying the percentage of BrdU-positive luminal epithelial cells in the mammary gland of the hormone treated WT, PRKO, and PR^d/d mouse. Scale bar in (a) and (b) applies to (c), (e), and (d), (f), respectively; LN denotes lymph node (a structural reference point).

Copyright

This image is from Fernandez-Valdivia R, Genesis 2010 Feb;48(2):106-13, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:158406

Associated Alleles

Symbol	Name
Pgr^tm1Bwo	progesterone receptor; targeted mutation 1, Bert W O'Malley
Pgr^tm4.2Lyd	progesterone receptor; targeted mutation 4.2, John P Lydon

Associated Genotypes

Allelic Composition	Genetic Background
Pgr^tm1Bwo/Pgr^tm1Bwo	involves: 129S7/SvEvBrd
Pgr^tm4.2Lyd/Pgr^tm4.2Lyd	involves: 129S4/SvJaeSor * 129S7/SvEvBrd * C57BL/6 * FVB/N