Phenotype Image Detail

Image

Caption

Altered lens differentiation in Tg(Pax6-HRAS*G12V)2044Ove/0 (Pax6-Ras) transgenic embryos. Immunohistochemistry (A-D, I, J, M-T) and in situ hybridizations (E-H, K, L) were performed on nontransgenic (NT) and Pax6-Ras transgenic embryos to detect expression of Pax6 (A, B), E-Cadherin (C, D, S, T), FoxE3 (E, F), Prox1 (G-J), Pitx3 (K, L) alpha-crystallin (M, N) and beta-crystallin (O-T). In panels A-D, I, J and M-R, antigen antibody complexes are in red and in panels S and T, green and red. In all these panels nuclei are stained blue with DAPI. In situ hybridizations were performed using ³⁵S-labeled riboprobes (E-H, K, L). Dark-field images were overlaid on respective bright-field images and silver grains were pseudocolored red. Upregulation of Prox1 (H, J) expression in a subset of Pax6-Ras transgenic lens epithelial cells suggests initiation of early fiber differentiation. Co-localization of beta-crystallin and ECadherin was seen in some of the Pax6-Ras transgenic lens cells (T, arrows). The staining in the vitreous (panel C, asterisk) is due to anti-mouse secondary antibody binding to IgGs in the blood vessels. White arrows in panel G, H and I point to the lens equatorial region where fiber differentiation is initiated. Green arrowheads point to lens epithelial cells (G-J).Abbreviations; C, corneal epithelium; Cj, conjunctival epithelium; le, lens epithelium; lf, lens fibers; nr, neural retina. The scale bar (I) represents 100 um in panels A-D, 25 um in panels I, J, S, T and 50 um in panels E-H, K-R.

Copyright

This image is from Burgess D, BMC Dev Biol 2010;10():13, an open-access article, licensee BioMed Central Ltd. J:158529

Associated Alleles

Symbol	Name
Tg(Pax6-HRAS*G12V)2044Ove	transgene insertion 2044, Paul A Overbeek

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Pax6-HRAS*G12V)2044Ove/0	involves: FVB/N