Phenotype Image Detail

Image

Caption

Abnormal proliferation, differentiation and no obvious change in apoptosis at the coronal suture in Fgfr2^tm2Ewj/Fgfr2⁺ Tg(EIIa-cre)C5379Lmgd/0 (+/P253R) mice. A, B) Immunohistochemical staining of BrdU shows decreased numbers and abnormal distribution of positive cells in mutants at E17.5 (arrow). C, D) ALP staining showed broad ALP domain and expansion of expression into the coronal suture of the mutants at E17.5 (arrow). E, F) Immunohistochemical stainingof Runx2 shows increased expression and abnormal differentiation at the osteogenic fronts at E17.5 (arrow). G, H) In situ hybridization of osteonectin shows accelebrated bone formation in mutants at E17.5. (I, J) TUNEL staining shows no clear difference in apoptosis between the mutant and wild-type at P5 (arrows). Panels A, C, E, G and I are from littermate controls. Panels B, D, F, H and J are the corresponding regions in mutant mice. (Scale bars: A-F = 50 um).

Copyright

This image is from Wang Y, BMC Dev Biol 2010;10():22, an open-access article, licensee BioMed Central Ltd. J:158773

Associated Alleles

Symbol	Name
Fgfr2^tm2Ewj	fibroblast growth factor receptor 2; targeted mutation 2, Ethylin Wang Jabs
Tg(EIIa-cre)C5379Lmgd	transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal

Associated Genotypes

Allelic Composition	Genetic Background
Fgfr2^tm2Ewj/Fgfr2^+ Tg(EIIa-cre)C5379Lmgd/0	B6.Cg-Fgfr2^tm2Ewj Tg(EIIa-cre)C5379Lmgd