Phenotype Image Detail

Image

Caption

Sel1l^{Gt(CA0017)Wtsi}/Sel1l^{Gt(CA0017)Wtsi} (-/-) pancreatic epithelial cells fail to commit to lineage precursors during the secondary transition. (A-D) Immunohistological analysis of the developing dorsal pancreas of wild-type (WT) and mutant embryos at E15, using the indicated antibodies. The genotypes of the pancreatic sections are indicated as "+/+" and "-/-", respectively. (A-B) PTF1a is expressed in the periphery of WT pancreatic epithelium marking the commitment of pancreatic epithelial cells into the exocrine lineage (A, white arrows); PTF1a expression is absent in the mutant pancreas (B). Up-regulation of PDX1 expression in a subset of epithelial cells in the core of WT pancreatic epithelium marks the commitment of pancreatic epithelial cells into the endocrine lineage (A, red arrows); whereas PDX1 is uniformly expressed in mutant pancreatic epithelial cells (B). (C-D) SOX9 is expressed in a small population of epithelial cells in WT pancreatic epithelium (C, red arrows); in contrast, SOX9 mostly co-localizes with PDX1 in mutant pancreatic epithelium (D, asterisks). Scale bar: 100 um.

Copyright

This image is from Li S, BMC Dev Biol 2010;10():19, an open-access article, licensee BioMed Central Ltd. J:158780

Associated Alleles

Symbol	Name
Sel1l^Gt(CA0017)Wtsi	sel-1 suppressor of lin-12-like (C. elegans); gene trap CA0017, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Sel1l^Gt(CA0017)Wtsi/Sel1l^Gt(CA0017)Wtsi	involves: 129P2/OlaHsd