Phenotype Image Detail

Image

Caption

Diminished insulin stores and mildly disorganized islets in Ero1b^{Gt(P077G11)Wrst}/Ero1b^{Gt(P077G11)Wrst} (i/i) mutant mice. (A) Hematoxylin- and eosin-stained pancreatic sections of 3-mo-old wild-type (WT) and homozygous mutant mice. (B) Pancreatic insulin content of mice as in A. Shown are the mean and SEM (n = 5; *, P < 0.001 by two-tailed t test). (C) Fluorescent micrographs of frozen section of pancreas from WT (+/+) and homozygous mutant mice (as in A) stained with antisera to insulin (Ins), glucagon, and the karyophilic dye DAPI. The bottom right panel in each genotype is an overlay of the three stains. Note the presence of glucagon-positive cells in the interior of the islet of the mutant. (D) Electron micrographs of insulin-producing Beta cells from islets of Langerhans of WT and mutant mice. Note the lack of ER dilation in the higher magnification views (right) of the mutant sample. Bars: (A and C) 100 um; (D, left) 2 um; (D, right) 0.5 um.

Copyright

This image is from Zito E, J Cell Biol 2010 Mar 22;188(6):821-32, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:158805

Associated Alleles

Symbol	Name
Ero1b^Gt(P077G11)Wrst	endoplasmic reticulum oxidoreductase 1 beta; gene trap P077G11, German Gene Trap Consortium

Associated Genotypes

Allelic Composition	Genetic Background
Ero1b^Gt(P077G11)Wrst/Ero1b^Gt(P077G11)Wrst	involves: 129S2/SvPas * C57BL/6