Phenotype Image Detail

Image

Caption

Reduced internuclear commissure in Robo3^tm1.1Ache/Robo3^tm1.1Ache Egr2^tm2(cre)Pch/Egr2⁺ (Krox20::cre;Robo3^lox/lox) mice. (A to I) show coronal hindbrain sections at the level of the abducens nucleus, visualized by Hb9 immunostaining (in A, D, E, F, and H). (A, B, and C) illustrate the projection of abducens axons (arrowheads) across the midline (dashed line) in Robo3+/- E13 embryos. Some GFP+ axons originate from the abducens nucleus (VI) and are immunoreactive for Robo3 (B and C). (D) The internuclear commissure (arrowhead) is also observed in P0 controls, following Dil injection at the level of the oculomotor nucleus III. (E) This commissure is almost completely absent in P0 Robo3^tm1.1Ache/Robo3^tm1.1Ache Egr2^tm2(cre)Pch/Egr2⁺ mice (arrowhead). (VIIn): Genu of facial nerve. (F to I) At E15, many neurofilament+ axons cross the midline at the VI level (arrowheads) in control embryo (F and G), whereas they are rare in the mutant embryo (H and I). Scale bars represent 100 um, except in (G and I), where they indicate 50 um.

Copyright

This image is from Renier N, PLoS Biol 2010;8(3):e1000325, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:158918

Associated Alleles

Symbol	Name
Egr2^tm2(cre)Pch	early growth response 2; targeted mutation 2, Patrick Charnay
Robo3^tm1.1Ache	roundabout guidance receptor 3; targeted mutation 1.1, Alain Chedotal

Associated Genotypes

Allelic Composition	Genetic Background
Egr2^tm2(cre)Pch/Egr2^+ Robo3^tm1.1Ache/Robo3^tm1.1Ache	involves: 129S2/SvPas