Phenotype Image Detail

Image

Caption

Phenotype of inferior olivary neurons in Ptf1a^{tm1.1(cre)Cvw}/Ptf1a⁺ Robo3^tm1.1Ache/Robo3^tm1.1Ache (Ptf1a::cre;Robo3^lox/lox) mice. (A-F) Coronal sections of P0 (A, B, D, and E) and E13.5 (C and F) hindbrain at the level of the inferior olive labeled with Brn3.2 (A-F) and calbindin (A and D). The structure of the inferior olivary nucleus is disorganized in the conditional mutant mice (compare [A and B] with [D and E]), and many of its subdivisions have an abnormal shape. The arrows in (D) show the position of dorsal cap of Kooy (DC in [A]) and the Beta nucleus (Beta in [A]) neurons, and the arrows in (E) indicate the disorganized principal olive (PO in [B]). (C and F) Brn3.2+ IO neurons do not cross the midline (arrow) in either control (C) or mutant (F) embryos. The arrowheads point to migrating LRN neurons. (G) is a 1.16-um-thick confocal image of Dil-labeled IO neurons in control P0 mouse with Hoechst counterstaining. (H and I) Schematic representation of the olivocerebellar projection in control (H) and mutant mice (I). In control, all IO neurons project across the ventral midline to the contralateral cerebellum (Cer) where their terminal arborization, the climbing fibers (CF), synapse on Purkinje cells. In mutant mice, most IO axons project into the ipsilateral cerebellar cortex. Scale bars represent 100 um, except in (G), where it indicates 20 um a, b, and c indicate the subnuclei a, b, and c, respectively, of the MAO, DAO, dorsal accessory olive; DC, Dorsal Cap of Kooy; MAO, medial accessory olive.

Copyright

This image is from Renier N, PLoS Biol 2010;8(3):e1000325, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:158918

Associated Alleles

Symbol	Name
Ptf1a^tm1.1(cre)Cvw	pancreas specific transcription factor, 1a; targeted mutation 1.1, Christopher VE Wright
Robo3^tm1.1Ache	roundabout guidance receptor 3; targeted mutation 1.1, Alain Chedotal

Associated Genotypes

Allelic Composition	Genetic Background
Ptf1a^tm1.1(cre)Cvw/Ptf1a^+ Robo3^tm1.1Ache/Robo3^tm1.1Ache	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ