Phenotype Image Detail

Image

Caption

Corneal differentiation in Tg(Pax6-HRAS*G12V)2044Ove/0 (Pax6-Ras) mice. In situ hybridizations (A, B) and immunohistochemistry (C-J) were performed on nontransgenic (NT) and Pax6-Ras transgenic embryos to detect expression of Hes1 (A, B), Keratin 12 (Krt12) (C, D), Keratin 14 (Krt14) (E, F), 14-3-3sigma (G, H) and Trp63 (I, J). For in situ hybridizations (A, B), dark-field images were overlaid on respective bright-field images and silver grains were colored red. For immunohistochemistry (C-J), antigen antibody complexes are in red and nuclei are stained blue with DAPI. Ras transgenic corneal epithelial cells show an expansion of the Hes1 expression domain (B) and reduced Krt12 expression (D). Abbreviations: C, corneal epithelium; Cj, conjunctival epithelium; le, lens epithelium; lf, lens fibers; nr, neural retina. The scale bar (I) represents 100 um in panels A-F, I, J and 50 um in panels G, H.

Copyright

This image is from Burgess D, BMC Dev Biol 2010;10():13, an open-access article, licensee BioMed Central Ltd. J:158529

Associated Alleles

Symbol	Name
Tg(Pax6-HRAS*G12V)2044Ove	transgene insertion 2044, Paul A Overbeek

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Pax6-HRAS*G12V)2044Ove/0	involves: FVB/N