Phenotype Image Detail

Image

Caption

Placentas of Arhgef11^tm1Sgkd/Arhgef11^tm1Sgkd Arhgef12^tm1Sgkd/Arhgef12^tm1Sgkd (Prg^-/-/Larg^-/-) embryos are less developed and poorly vascularized. A, untreated yolk sacs from wild-type (WT) and double knock-out E10 embryos. Blood-containing vessels are present in the WT yolk sac (arrows). B, whole mount PECAM-1 staining of yolk sacs from WT and double knock-out E10 embryos. Organized, mature vessels are present in the yolk sacs of WT embryos (arrows), versus disorganized and immature vasculature (arrowheads) in double-deficient embryos. C, histological appearance of hematoxylin and eosin (H&E)-stained placental tissues of Arhgef11^tm1Sgkd/Arhgef11^tm1Sgkd (Prg^-/-/Larg^+/+, left panels) and double-deficient (Prg^-/-/Larg^-/-, right panels) embryos at E9.5 (top panels) and E10.5 (bottom panels). The thickness of the placental labyrinth (double-headed arrows between the dotted lines) is reduced indouble-deficient tissues. At E10.5, the complexity of the labyrinth, characterized by the number of fetal vessels (arrows) and lacunae filled with maternal blood (arrowheads) within the labyrinth is markedly reduced in double-deficient, compared with Arhgef11^tm1Sgkd/Arhgef11^tm1Sgkd tissues.

Copyright

This image is from Mikelis CM, J Biol Chem 2013 Apr 26;288(17):12232-43 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:198274

Associated Alleles

Symbol	Name
Arhgef11^tm1Sgkd	Rho guanine nucleotide exchange factor 11; targeted mutation 1, Silvio Gutkind
Arhgef12^tm1Sgkd	Rho guanine nucleotide exchange factor 12; targeted mutation 1, Silvio Gutkind

Associated Genotypes

Allelic Composition	Genetic Background
Arhgef11^tm1Sgkd/Arhgef11^tm1Sgkd Arhgef12^tm1Sgkd/Arhgef12^tm1Sgkd	involves: 129 * C57BL/6J