Phenotype Image Detail

Image

Caption

Apoptotic cell death of primordial germ cells (PGCs) during migration in Mad2l2^tm1Ymu/Mad2l2^tm1Ymu (Rev7^-/-) embryos. A, immunohistochemical analysis of whole mount mouse embryos to identify PGCs during migration. Wild-type (WT, Rev7^+/+) and mutant embryos at E8.5, E9.5, and E10.5 were whole mount immunostained using the anti-Oct4 antibody. Images were obtained under a stereomicroscope. B, immunohistochemical analysis of paraffin sections to detect PGCs and apoptotic cells. Sections around the genital ridges of WT and mutant embryos at E11.5 and E13.5 were immunostained with the anti-Oct4 antibody to detect PGCs (left and middle panels). Those of WT and mutant embryos at E11.5 were immunostained with the anti-cleaved caspase-3 antibody to detect apoptotic cells (right panels). Sections of E11.5 embryos used for Oct4 and cleaved caspase-3 staining (left and right panels) were serial sections. Arrows indicate cells positive for both Oct4 and cleaved caspase-3. Scale bars, 100 um (left and right panels) and 200 um (middle panels). C, fluorescence staining to demonstrate PGC apoptosis. Sections around the hindgut of WT and mutant mouse embryos at E9.5 were subjected to immunostaining with the anti-Oct4 antibody, TUNEL staining, and nuclear staining with DAPI. Merged images are also shown. Arrowheads indicate cells positive for both Oct4 and TUNEL. Scale bars, 25 um.

Copyright

This image is from Watanabe N, J Biol Chem 2013 Apr 12;288(15):10459-71 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:198275

Associated Alleles

Symbol	Name
Mad2l2^tm1Ymu	MAD2 mitotic arrest deficient-like 2; targeted mutation 1, Yoshiki Murakumo

Associated Genotypes

Allelic Composition	Genetic Background
Mad2l2^tm1Ymu/Mad2l2^tm1Ymu	involves: 129 * C57BL/6J