Phenotype Image Detail

Image

Caption

D, disrupted axoneme in Ttll5^tm1.1N/Ttll5^tm1.1N (TM) sperm tails. Electron microscopy detected the canonical arrangement of nine peripheral microtubule doublets, and the larger associated, more peripheral outer dense bodies or fibers, surrounding a central doublet (9 + 2) that were intact in transverse and longitudinal sections in 100% of normal sperm tails. The top left panel is from wild-type (WT) sperm, with the numbering of the nine microtubule doublets. In homozygous mutant sperm, doublet 4 is missing from the 9 + 2 formation in virtually all of the axonema (see top left panel of TM pictures for numbering, with the red number 4 indicating the position of missing doublet 4 and white arrows for other examples). Severe disruption of microtubule polymers (arrows in bottom row) was observed in transverse sections. Occasionally, more severe defects were observed (middle row). The more peripheral dense fibers were generally indistinguishable from normal controls except that the outer dense fiber paired with doublet 4 usually disappears with doublet 4. The samples were independently obtained from three biological samples. Scale bars, 0.2 um.

Copyright

This image is from Lee GS, J Biol Chem 2013 May 24;288(21):15167-80 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:198583

Associated Alleles

Symbol	Name
Ttll5^tm1.1N	tubulin tyrosine ligase-like family, member 5; targeted mutation 1.1, National Institutes of Health

Associated Genotypes

Allelic Composition	Genetic Background
Ttll5^tm1.1N/Ttll5^tm1.1N	involves: 129S6/SvEvTac * C57BL/6NTac * FVB/N