Phenotype Image Detail

Image

Caption

Skeletal defects in Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor^tm1Fan Tg(T-cre)1Lwd/0 (mutant) mice during somitogenesis. (A) Control (left) and mutant (right) neonates (P0); dotted lines mark the postures. (B-H) Skeletal preparations stained with Alizarin red and Alcian blue. (B) Entire skeletons of control (left) and mutant (right). Control (C, E, and G) and mutant (D, F, and H) axial skeletons. (C and D) dorsal view; (E and F) ventral view; and (G and H) magnified views of the rectangular area in E and F, respectively. Arrowhead indicates bifurcation of rib (marked as "R"), and arrow indicates fusion of the lamina. White arrowheads indicate fusions of pedicles (marked as "P"), and the bracket indicates fusions of vertebral bodies (VB). Control in G has intervertebral discs (IVD), and the black arrowhead in the mutant indicates a loss of intervertebral discs (H). (I-L) Whole-mount in situ hybridization (WISH) analysis of Tbx18 (I and J) and Uncx4.1 (K and L) in controls (I and K) and mutants (J and L).

Copyright

This image is from Lopez TP, Proc Natl Acad Sci U S A 2013 May 28;110(22):E2019-27. Copyright 2013 National Academy of Sciences, U.S.A. J:197427

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1Fan	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Chen-Ming Fan
Tg(T-cre)1Lwd	transgene insertion 1, Mark Lewandoski

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor^tm1Fan Tg(T-cre)1Lwd/0	involves: C3H * C57BL/6 * C57BL/6J