Phenotype Image Detail

Image

Caption

Osteoclast (OC) differentiation is increased in Nlrp3^tm3.1Hhf/Nlrp3⁺ (NOMID) cells. Unfractionated bone marrow cells (A) or bone marrow-derived macrophages (BMM) previously cultured for 3 days in the presence of M-CSF(B) were induced to differentiate into OC in the presernce of M-CSF and 100 ng/ml RANKL. (C) Co-cultures of wild-type (WT) or NOMID (NOM) BMM and WT bone marrow stromal cells (BMSC) were carried out in the presence of 10 nM dexamethasone and 1 nM 1,25(OH)2 vitamin D3 for 5-7 days. The cultures were stained for TRAP activity, and the number of OC (cells stained in red with >3 nuclei, arrow) were counted manually. The pictures were taken at the same magnification (X4) for both genotypes. The data show thatNOMID cells formed more OC than WT cells. Determinations were performed in triplicate and expressed as the mean +/- S.E.M. Results are representative of at least three independent experiments. **P<0.007.

Copyright

This image is from Bonar SL, PLoS One 2012;7(4):e35979, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:202147

Associated Alleles

Symbol	Name
Nlrp3^tm3.1Hhf	NLR family, pyrin domain containing 3; targeted mutation 3.1, Hal M Hoffman

Associated Genotypes

Allelic Composition	Genetic Background
Nlrp3^tm3.1Hhf/Nlrp3^+	involves: 129