Phenotype Image Detail

Image

Caption

Gli2 activation, but not Gli3 de-repression, mediates Hedgehog function in the developing gut. A-E, histology of the control, Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ Gli3^tm1Alj/Gli3^tm1Alj (Nkx3.2^Cre;Gli3^C/C), Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ Gt(ROSA)26Sor^{tm1(Gli2)Jmao}/Gt(ROSA)26Sor⁺ (Nkx3.2^Cre;R26^Gli2), Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ Smo^tm2Amc/Smo^tm2Amc Gli3^tm1Alj/Gli3^tm1Alj (Nkx3.2^Cre;Smo^C/C;Gli3^C/C), and Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ Smo^tm2Amc/Smo^tm2Amc Gt(ROSA)26Sor^{tm1(Gli2)Jmao}/Gt(ROSA)26Sor⁺ (Nkx3.2Cre;Smo^C/C;R26^Gli2) intestine at E18.5. F-I, epithelial and mesenchymal development in the Nkx3.2^Cre;Smo^C/C;R26^Gli2 intestine at E18.5. Immunohistochemical staining of Beta-catenin (F), Ki67 (G), SMA (H), and Beta-tubulin (I) in the mutant embryos is shown. Gli2 expression from the Rosa26 locus completely rescued the Smo-null intestinal phenotypes. Histological analyses of E18.5 embryos revealed that intestinal development in the Nkx3.2^Cre;Smo;R26^Gli2 embryos is nomral (E). We observed the rescue of mesenchymal growth, smooth muscle development, and intestinal epithelial differentiation (F-I).

Copyright

This image is from Huang H, J Biol Chem 2013 Jun 14;288(24):17589-96 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:199664

Associated Alleles

Symbol	Name
Gli3^tm1Alj	GLI-Kruppel family member GLI3; targeted mutation 1, Alexandra L Joyner
Gt(ROSA)26Sor^tm1(Gli2)Jmao	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Junhao Mao
Nkx3-2^tm1(cre)Wez	NK3 homeobox 2; targeted mutation 1, Warren E Zimmer
Smo^tm2Amc	smoothened, frizzled class receptor; targeted mutation 2, Andrew P McMahon

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1(Gli2)Jmao/Gt(ROSA)26Sor^+ Nkx3-2^tm1(cre)Wez/Nkx3-2^+	involves: 129S7/SvEvBrd
Gt(ROSA)26Sor^tm1(Gli2)Jmao/Gt(ROSA)26Sor^+ Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2^+	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ
Gli3^tm1Alj/Gli3^tm1Alj Nkx3-2^tm1(cre)Wez/Nkx3-2^+	involves: 129S6/SvEvTac * 129S7/SvEvBrd
Gli3^tm1Alj/Gli3^tm1Alj Nkx3-2^tm1(cre)Wez/Nkx3-2^+ Smo^tm2Amc/Smo^tm2Amc	involves: 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ