Phenotype Image Detail

Image

Caption

(D) Electron micrographs at three different magnifications in rhomboid muscle fibers from Fat1^{Gt(KST249)Byg}/Fat1^{Gt(KST249)Byg} Tg(Myl1-lacZ)1Ibdml/0 (Fat1^LacZ/LacZ) adult mice at early symptomatic stages (6-15% weight loss) show fragmentation of the myofiber architecture and loss of t-tubule integrity. In wild-type (WT) myofibers, t-tubules (purple arrows) are visible between myofibrils, precisely aligned on either side of each Z-band, at a position coinciding with the end of the myosin filaments. By contrast, in dystrophic fibers from mutant mice, the general disorganization correlated with missing (stars), mis-oriented, mis-aligned (orange arrows), or fragmented (red arrows) triads. An increased distance (indicated as blue double arrowed bar) between the sarcolemma and contractile apparatus is observed in mutant muscles, compared to WT, indicating a loss of the tight association between the contractile apparatus and the sarcolemma. Scale bars: 15 um; (D1, 2) 5 um; (D3, 4) 0.5 um; (D5, 6) 0.2 um.

Copyright

This image is from Caruso N, PLoS Genet 2013 Jun;9(6):e1003550, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199157

Associated Alleles

Symbol	Name
Fat1^Gt(KST249)Byg	FAT atypical cadherin 1; gene trap KST249, BayGenomics
Tg(Myl1-lacZ)1Ibdml	transgene insertion 1, Developmental Biology Institue of Marseilles-Luminy

Associated Genotypes

Allelic Composition	Genetic Background
Fat1^Gt(KST249)Byg/Fat1^Gt(KST249)Byg Tg(Myl1-lacZ)1Ibdml/0	involves: 129P2/OlaHsd