Phenotype Image Detail

Image

Caption

Extent of muscle wasting in adult Fat1^{Gt(KST249)Byg}/Fat1^{Gt(KST249)Byg}Tg(Myl1-lacZ)1Ibdml/0 (Fat1^LacZ/LacZ) mice at presymptomatic and symptomatic stages of phenotype progression. Muscle dissection in adult wild-type (WT) (B1, 4) and mutant mice at presymptomatic (B2, 5) or advanced symptomatic (B3, 6; 28% weight loss) stages reveals a pronounced reduction in volume and thickness of the cutaneous maximus (CM) muscle at both stages, while Gastrocnemius muscle in the hindlimb only show pronounced wasting at later stages or phenotype progression. Because of the tight association with the skin of its caudal, the CM cannot easily be dissected for weight measurements. In pictures in B1, 2, 3, this skin-associated caudal part was therefore arbitrarily cut, preventing fair assessment of its true volume. (D) Histological section of Cutaneous maximus muscles from adult WT and mutant mice at presymptomatic stage, lightly stained with Toluidin Blue, showing reduced thicknessas well as reduced myofiber density, reduced myofiber diameter, and infiltration with connective tissue (bottom, higher magnification). Scale bars: (B1-6) 4 mm; (B7-9) 50 um.

Copyright

This image is from Caruso N, PLoS Genet 2013 Jun;9(6):e1003550, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199157

Associated Alleles

Symbol	Name
Fat1^Gt(KST249)Byg	FAT atypical cadherin 1; gene trap KST249, BayGenomics
Tg(Myl1-lacZ)1Ibdml	transgene insertion 1, Developmental Biology Institue of Marseilles-Luminy

Associated Genotypes

Allelic Composition	Genetic Background
Fat1^Gt(KST249)Byg/Fat1^Gt(KST249)Byg Tg(Myl1-lacZ)1Ibdml/0	involves: 129P2/OlaHsd