Phenotype Image Detail

Image

Caption

Examples of neuromuscular junction (NMJ) denervation and atrophy in Fat1^{Gt(KST249)Byg}/Fat1^{Gt(KST249)Byg} Tg(Myl1-lacZ)1Ibdml/0 (Fat1^LacZ/LacZ) mice. NMJs were visualized in wild-type (WT) (A1-A3) and mutant (B1-D3) rhomboid muscles by immunolabelling AChR clusters with alpha-bungarotoxin (alpha-BTX, green), and nerve endings with neurofilaments (NF, red). Each synapse is shown with separate alpha-BTX-green (1) and NF-red channels (2) separately, and with a merge image of both channels (3). In WT synapses, the NF-positive axon endplate overlaps with all circumvolutions of the bretzel-like shaped alpha-BTX-positive postsynaptic area. A portion of axon proximal to the NMJ is intensely stained with NF (white stars in all panels). By contrast, mutant synapses display reduced alpha-BTX-positive surface, with fragmentation, interruptions, swelling,.or absence of the axon endplate, although the NF-positive axon (white stars) is still detectable.

Copyright

This image is from Caruso N, PLoS Genet 2013 Jun;9(6):e1003550, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199157

Associated Alleles

Symbol	Name
Fat1^Gt(KST249)Byg	FAT atypical cadherin 1; gene trap KST249, BayGenomics
Tg(Myl1-lacZ)1Ibdml	transgene insertion 1, Developmental Biology Institue of Marseilles-Luminy

Associated Genotypes

Allelic Composition	Genetic Background
Fat1^Gt(KST249)Byg/Fat1^Gt(KST249)Byg Tg(Myl1-lacZ)1Ibdml/0	involves: 129P2/OlaHsd