Phenotype Image Detail

Image

Caption

Fat1 ablation in trunk premigratory muscle precursors under Pax3-cre reproduces the scapulohumeral muscle shape phenotypes of the constitutive mutants. Muscle anatomy was visualized at E14.5 (A, B) and E15.5 (C), by X-Gal staining in Fat1^tm1Fhel/Fat1^tm1Fhel Tg(Myl1-lacZ)1Ibdml/0 (Fat1^Fln/Fln; MLC3f-2E) and Fat1^tm1Fhel/Fat1^tm1Fhel Pax3^tm1(cre)Joe/Pax3⁺ Tg(Myl1-lacZ)1Ibdml/0 (Fat1^Fln/Fln; Pax3cre/⁺; MLC3f-2E) embryos. Mild phenotypes can be detected in mutant embryos in the face (reduced occip. Frontalis muscle, and zygomatics), and through appearance of misplaced muscle fibers between Trapezius Cervicalis and Trapezius Thoracis, frequently unilateral or asymmetric (red arrow). While Pax3-cre driven recombination in Fat1^Fln/Fln; Pax3cre/⁺; MLC3f-2E embryos does not cause any worsening in muscle shape and size in the face, abnormalities can be seen in the scapulohumeral region, such as the appearance of an additional muscle, in an ectopic position reminiscent of that seen in Fat1^tm1.2Fhel/Fat1^tm1.2Fhel Tg(Myl1-lacZ)1Ibdml/0 (Fat1^{deltaTM/deltaTM}) embryos, without insertion of its extremity between the spinodeltoid and the Triceps brachii muscles.

Copyright

This image is from Caruso N, PLoS Genet 2013 Jun;9(6):e1003550, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199157

Associated Alleles

Symbol	Name
Fat1^tm1.2Fhel	FAT atypical cadherin 1; targeted mutation 1.2, Francoise Helmbacher
Fat1^tm1Fhel	FAT atypical cadherin 1; targeted mutation 1, Francoise Helmbacher
Pax3^tm1(cre)Joe	paired box 3; targeted mutation 1, Jonathan A Epstein

Associated Genotypes

Allelic Composition	Genetic Background
Fat1^tm1Fhel/Fat1^tm1Fhel Pax3^tm1(cre)Joe/Pax3^+ Tg(Myl1-lacZ)1Ibdml/0	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * SJL