Phenotype Image Detail

Image

Caption

Nbeal2^tm1Lex/Nbeal2^tm1Lex (-/-) platelets show defective adhesion, thrombus formation, and procoagulant phosphatidylserine (PS) exposure under flow. (A) Severely impaired adhesion and thrombus formation of mutant plateletson collagen under flow at a shear rate of 1,700 s^-1. Representative phase-contrast (bright field [BF]) and fluorescence images are shown as well as mean surface coverage and relative platelet deposition, as measured by integrated fluorescent intensity (IFI) per mm² +/- SD (n = 4 mice per group). Scale bar: 50 um. (B) Representative phase-contrast (BF) and fluorescence images of mutant platelets stained with Annexin A5-DyLight 488 perfused over a collagen-coated surface at a shear rate of 1,000s^-1. Scale bar: 65 um. (C) Procoagulant index represents the ratio of Annexin A5-positive cells to surface coverage (n = 5 mice per group). (D) Highly diluted washed platelets were stimulated with 1 ug/ml convulxin, 20 ug/ml CRP, or 20 ug/ml CRP plus 0.1U/ml thrombin, and the percentage of Annexin A5-positive cells was determined by flow cytometry (n = 5). Data are representative of 3 independent experiments. *P<0.05; ***P<0.001.

Copyright

This image is from Deppermann C, J Clin Invest 2013 Jul 1;(): and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:201413

Associated Alleles

Symbol	Name
Nbeal2^tm1Lex	neurobeachin-like 2; targeted mutation 1, Lexicon Pharmaceuticals
Nbeal2^tm1Lex	neurobeachin-like 2; targeted mutation 1, Lexicon Pharmaceuticals

Associated Genotypes

Allelic Composition	Genetic Background
Nbeal2^tm1Lex/Nbeal2^tm1Lex	involves: 129S5/SvEvBrd * C57BL/6J