Phenotype Image Detail

Image

Caption

Retinal microglial activation in different ages of Ccl2^tm1Rol/Ccl2^tm1Rol Cx3cr1^tm1Litt/Cx3cr1^tm1Litt (DKO) mice. (A-F, K-M) Retinal flatmounts from double knockout (DKO) mice were stained for isolectin B4 (red) and examined by confocal microscopy. (A, C, E) Confocal images from inner plexiform layer (IPL). (B, D, F) Confocal images from outer plexiform layer (OPL). (A, B) 3-month old. (C, D), 18-month old. (E, F) 10-month+light -treatment. (G) The number of microglial cells in different groups of mice. (H) High magnification image showing activated microglia from light-treated mice. (I) High magnification image showing a naive resident microglia from a 3-month old DKO mouse. (J) High magnification image showing a CD11b+ naive resident microglia from a 3-month old wild-type mouse. (K-M) Z-axils reconstruction of confocal z-stack images from a 3-month old (K), an 18-month old (L), and 10-month+light mutant (M) mouse retina. (N-Q) RPE/choroidal flatmounts showing subretinal macrophages in 3-month old (N), an 18-month old (O), and 10-month+light (P, Q) mutant eyes. RPE cells were stained with rhodamine-phalloidin (red). (R) The number of subretinal macrophages in different groups of mice. *,P<0.05; **<0.01, n = 6. GL, Ganglion layer; PL, photoreceptor layer. Scale bar = 50 um.

Copyright

This image is from Chen M, PLoS One 2013;8(4):e61381, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:200877

Associated Alleles

Symbol	Name
Ccl2^tm1Rol	C-C motif chemokine ligand 2; targeted mutation 1, Barrett J Rollins
Cx3cr1^tm1Litt	C-X3-C motif chemokine receptor 1; targeted mutation 1, Dan R Littman

Associated Genotypes

Allelic Composition	Genetic Background
Ccl2^tm1Rol/Ccl2^tm1Rol Cx3cr1^tm1Litt/Cx3cr1^tm1Litt	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6J