Phenotype Image Detail

Image

Caption

Gt(ROSA)26Sor^{tm1(Tbx1/GFP)Bem} (Tbx1-GFP) can patially rescue heart phenotypes in Tbx1^tm6(cre)Bld/Tbx1^tm1Bld (Tbx1^Cre/-) mice. Histological transverse sections through the heart at E14.5. (A) Tbx1^tm6(cre)Bld/Tbx1^tm6(cre)Bld mice are functionally null and have persistent truncus arteriosus (PTA) and ventricular septal defects (VSD). Tbx1^Cre/+;Tbx1-GFP^flox/+ mutants are viable with normal heart histology. (B) Tbx1^tm6(cre)Bld/Tbx1^tm1Bld Gt(ROSA)26Sor^{tm1(Tbx1/GFP)Bem}/Gt(ROSA)26Sor⁺ (Tbx1^Cre/-;Tbx1-GFP^flox/+) mutants exhibit complete rescue of outflow tract septation although half of the embryos have double outlet right ventricle (DORV). (C) Tbx1^tm6(cre)Bld/Tbx1^tm1Bld Gt(ROSA)26Sor^{tm1(Tbx1/GFP)Bem}/Gt(ROSA)26Sor^{tm1(Tbx1/GFP)Bem} (Tbx1^Cre/-;Tbx1-GFP^flox/flox) mutants all have VSD, primarily with PTA and one example of DORV.

Copyright

This image is from Freyer L, BMC Dev Biol 2013 Aug 23;13(1):33, an open-access article, licensee BioMed Central Ltd. J:204435

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1(Tbx1/GFP)Bem	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Bernice E Morrow
Tbx1^tm1Bld	T-box 1; targeted mutation 1, Antonio Baldini
Tbx1^tm6(cre)Bld	T-box 1; targeted mutation 6, Antonio Baldini

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1(Tbx1/GFP)Bem/Gt(ROSA)26Sor^+ Tbx1^tm1Bld/Tbx1^tm6(cre)Bld	involves: 129S7/SvEvBrd * C57BL/6 * SJL
Gt(ROSA)26Sor^tm1(Tbx1/GFP)Bem/Gt(ROSA)26Sor^tm1(Tbx1/GFP)Bem Tbx1^tm1Bld/Tbx1^tm6(cre)Bld	involves: 129S7/SvEvBrd * C57BL/6 * SJL