Phenotype Image Detail

Image

Caption

Hearts from adult Ngf^tm1.1Blhe/Ngf⁺ (proNgf-HA/+) mice exhibit microvascular damage and hearts from juvenile mutant mice show early signs of endothelial cell activation and microvascular damage. (A-C) Transmission electron microscopic images of hearts from 4-month-old wild-type (Ngf+/+), Ngf^tm1Gne/Ngf⁺ (Ngf+/-), and Ngf^tm1.1Blhe/Ngf⁺ (proNgf-HA/+) mice, respectively. Arrowhead, microvascular endothelial cell with fragmented plasma membrane. (D-F) Normal H and E histology of 1-month-old wild-type, Ngf+/-, and proNgf-HA/+ hearts. (G-I) Representative transmission electron microscope images of hearts from 1-month-old wild-type, Ngf+/-,and proNgf-HA/+ mice. Microvascular endothelial cells from proNgf-HA/+ mice exhibit filamentous membrane projections into the vessel lumen (I, arrow). In the proNgf-HA/+ myocardium, ~50% of microvascular endothelial cells examined showed signs of activation as well as perivascular edema (I, asterisk). Additionally, a loss of apposition in the processes of pericyte with endothelial cells is observed (arrowhead). Bars: (A-C and G-I) 2 um; (D-F) 100 um. n = 2/genotype.

Copyright

This image is from Siao CJ, J Exp Med 2012 Nov 19;209(12):2291-305, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:190893

Associated Alleles

Symbol	Name
Ngf^tm1.1Blhe	nerve growth factor; targeted mutation 1.1, Barbara L Hempstead

Associated Genotypes

Allelic Composition	Genetic Background
Ngf^tm1.1Blhe/Ngf^+	B6.Cg-Ngf^tm1.1Blhe