Phenotype Image Detail

Image

Caption

Hair cell basolateral and hair bundle properties in Eps8l2^tm1Wma/Eps8l2^tm1Wma (Eps8L2-KO) mice. (A and B) Currents from a control and a mutant adult P96 inner hair cell (IHC), respectively, were elicited by depolarizing voltage steps in 10-mV nominal increments from the holding potential of -64 mV to the various test potentials shown by some of the traces. (C) Average size of the delayed rectifier (IK) and the BK (IK,f) currents (15) from P18 and P96 IHCs. The current carried by KCNQ4 channels (IK,n) was also similarly expressed in IHCs from both genotypes (control: -262 64 pA, n = 9; Eps8L2 KO: KO -273 54 pA, n = 11; P18-P96). (D) Distribution of the motor protein prestin (green) and the KCNQ4 channels (red) in control and mutant adult (4.5 mo old) outer hair cells (OHCs). Prestin is a major component of the OHC lateral membrane and it is crucial for sustaining OHC voltage-dependent electromotile activity. (Scale bar, 10 um.) Nuclei stained by DAPI (blue). (E and F) SEM images showing the presence of hair bundles (i.e., hair cells) in control and mutant P90 cochleae. (Scale bar, 10 um.) (G) SEM image showing tip links (arrow) in a P90 OHC from a mutant mouse. Right shows a high-magnification image of tip links. [Scale bars, 1.5 um (Left) and 200 nm (Right).]

Copyright

This image is fromFurness DN, Proc Natl Acad Sci U S A 2013 Aug 20;110(34):13898-903. Copyright2013 National Academy of Sciences, U.S.A. J:200669

Associated Alleles

Symbol	Name
Eps8l2^tm1Wma	EPS8-like 2; targeted mutation 1, Walter Marcotti

Associated Genotypes

Allelic Composition	Genetic Background
Eps8l2^tm1Wma/Eps8l2^tm1Wma	B6.129P2-Eps8l2^tm1Wma