Phenotype Image Detail

Image

Caption

Embryonic abnormalities and craniofacial dysmorphology in Gtf2i^tm1Vcam/Gtf2i^tm1Vcam (deltaex2/deltaex2) mutant mice. A. H&E staining, Ki67 labeling and TUNEL assay. Sections from normal (top) and abnormal (bottom) E7.5 embryos derived from crosses between heterozygous females and homozygous males. Abnormal embryos lacking detectable embryonic layers by H&E staining are likely to correspond to nonviable homozygous embryos. Normal embryos displayed high levels of Ki67-positive cells while they are not detectable in abnormal embryos. No differences were observed in the TUNEL staining. B. Morphologic abnormalities in mutant mice at 8 weeks of age. (Left) The histogram shows the relative average distance from the tip of the nose to the proximal occipital suture (n = 10 per genotype). Pair wise comparisons revealed statistically significant differences between genotypes (Tukey test; P = 0.003 and P = 0.048 for homozygous and heterozygous relative to wild-type, respectively). (Right) representative photograph of the dissected cranial structure per genotype is shown. A shorter snout could be clearly appreciated in the homozygous mice. wild-type, open; heterozygous, grey; and homozygous, black squares.

Copyright

This image is from Lucena J, BMC Med Genet 2010;11():61, an open-access article, licensee BioMed Central Ltd. J:204278

Associated Alleles

Symbol	Name
Gtf2i^tm1Vcam	general transcription factor II I; targeted mutation 1, Victoria Campuzano

Associated Genotypes

Allelic Composition	Genetic Background
Gtf2i^tm1Vcam/Gtf2i^tm1Vcam	involves: 129S1/Sv * 129X1/SvJ * CD-1