Phenotype Image Detail

Image

Caption

Sox2-Cre-mediated conditional knockout revealed that embryonic Ubr4 (p600) is essential for heart development. (A) Impaired interventricular septum formation in Ubr4^tm1.2Nkt/Ubr4^tm1.2Nkt Edil3^{Tg(Sox2-cre)1Amc}/0 (p600 cKO) heart. From H&E stained transverse cross-sections of E12.5 embryos, the interventricular septum areas are shown. In the control Ubr4^tm1.2Nkt/Ubr4⁺ Edil3^{Tg(Sox2-cre)1Amc}/0 (p600^+/-) heart (left), the interventricular septum (IVS) is fused with inferior atrioventricular endocardial cushions (IC), separating the left and right ventricles. On the other hand, mutant heart has developmental defects of the interventricular septum. Scale bars indicate 500 um. (B) The control (left) and mutant (right) heart at E13.5. Scale bars indicate 200 um. (C) Magnified images of ventricular wall. Magnified areas are indicated as boxes in (B). Scale bars indicate 100 um. (D) Relative cell numbers of the ventricular wall in the mutant and control hearts at E13.5. Cell numbers of ventricular wall areas were estimated from the several serial sections and shown as relative values. The statistical significance of the difference was calculated with Student's t-test. * shows p-value <0.05.

Copyright

This image is from Nakaya T, PLoS One 2013;8(6):e66269, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:203460

Associated Alleles

Symbol	Name
Edil3^Tg(Sox2-cre)1Amc	EGF-like repeats and discoidin I-like domains 3; transgene insertion 1, Andrew P McMahon
Ubr4^tm1.2Nkt	ubiquitin protein ligase E3 component n-recognin 4; targeted mutation 1.2, Yoshihiro Nakatani

Associated Genotypes

Allelic Composition	Genetic Background
Ubr4^tm1.2Nkt/Ubr4^tm1.2Nkt Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S6/SvEvTac * C57BL/6 * CBA * SJL