Phenotype Image Detail

Image

Caption

Histopathology in Trpv4^tm1Rck/Trpv4^tm1Rck (Trpv4-/-) and control mice in response to UVB. This bar diagram shows the relative level of knockdown of Trpv4 in comparison with wild-type (WT) of UVB-exposed skin samples. An adjacent sample of hind-paw skin was RNA extracted at 48 h post exposure and subjected to Trpv4 qRT-PCR; pooled WT mRNA values from 10 mice were set as 100%. (B) Light microscopic analyses of 1-um semithin sections from mutant and WT mice. Normal skin is shown in the upper row for both genotypes in the unstimulated state, presence of epidermal and dermal tissue damage in WT control vs. absence thereof in mutant when exposing the skin to UVB, sampling conducted at 48 h. (C) Ultrastructural analyses of mutant and WT control mice. (a, a') and (b, b') WT and mutant mice show normal skin morphology with intact epidermis (Epi) in the absence of UVB stimulation. a' and b' show higher magnification of basal layer (BL) cells. (c, c') Damaged epidermis with vacuolization (Inset) and granulocyte (GR) (neutrophil) infiltrate (c'). (d, d') Normal epidermal and dermal ultrastructure in mutant mice exposed to UVB. Der, dermis; NT, nerve terminals. Dotted lines indicate the dermal-epidermal boundary. (Scale bars, 10 um for a, b, c, c' and d and 2 um for the other micrographs.) (D) IL-6 up-regulation in epidermal keratinocytes in response to UVB depends on Trpv4; findings from mutant and WT control mice. Fluorescent micrographs from mutant and WT control skin, unexposed and exposed to UVB are shown. Note strong IL-6 signal in WT, exposed to UVB, and low signal in mutants for both nonexposed and UVB-exposed states. Also note IL-6-expressing innervating peripheral nerve endings in the dermis.

Copyright

This image is from Moore C, Proc Natl Acad Sci U S A 2013 Aug 20;110(34):E3225-34. Copyright 2013 National Academy of Sciences, U.S.A. J:200749

Associated Alleles

Symbol	Name
Trpv4^tm1Rck	transient receptor potential cation channel, subfamily V, member 4; targeted mutation 1, J M Friedman

Associated Genotypes

Allelic Composition	Genetic Background
Trpv4^tm1Rck/Trpv4^tm1Rck	Not Specified