Phenotype Image Detail

Image

Caption

Inhibition of vascular growth and production of excess tip cells in Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}/Igs1⁺ Tg(Six3-cre)69Frty/0 (Z/Edn2;Six3-Cre) retinas. (A-D) Flat-mount retinas from P6 control and mutant mice immunostained for PECAM1 (ECs) and PDGFR-alpha (astrocytes). The optic disk is at the bottom of each image. Dashed lines mark the edge of the developing retina. (Scale bar: 200 um.) (E) Quantification of the fraction of retinal surface area covered by blood vessels and astrocytes in control and mutant retinas at P3, P6, and P8. For each retina, distances were measured from the optic disk to the edge of the developing vasculature, the astrocyte network, and the retina along three directions, and the ratios were averaged. The histograms show the mean and SD ofmeasurements from the indicated number of retinas (n) with corresponding P values. (F and G) High magnification images of the leading edge of the developing vasculature in flat-mount control and mutant retinas at P6 stained as in A-D. In both cases, ECs grow along the astrocyte network. (Scale bar: 100 um.) (H and I) High-magnification images of ECs at the leading edge of the developing vasculature in flat-mount control and mutant retinas at P6 immunostained for PECAM1. (H) In control retinas, filopodia (arrowheads) are confined to tip cells at the front. (I) In mutant retinas, filopodia are on ECs at the vascular front and within the vascular plexus. (Scale bar: 50 um.)

Copyright

This image is from Rattner A, Proc Natl Acad Sci U S A 2013 Oct 1;110(40):E3830-E3839. Copyright 2013 National Academy of Sciences, U.S.A. J:201133

Associated Alleles

Symbol	Name
Igs1^tm11(CAG-Bgeo,-Edn2)Nat	intergenic site 1; targeted mutation 11, Jeremy Nathans
Tg(Six3-cre)69Frty	transgene insertion 69, Yasuhide Furuta

Associated Genotypes

Allelic Composition	Genetic Background
Igs1^tm11(CAG-Bgeo,-Edn2)Nat/Igs1^+ Tg(Six3-cre)69Frty/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2