Phenotype Image Detail

Image

Caption

In developing Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}/Igs1⁺ Tg(Six3-cre)69Frty/0 (Z/Edn2;Six3-Cre) retinas, large regions of the endothelial cell (EC) network are not perfused. (A and B) Retina flat mounts from (A) control and (B) mutant retinas at P6 after intracardiac injection of Alexa595 GS-lectin and postfixation permeabilization and labeling with Alexa488 GS-lectin. Right shows an enlargement of the region at the vascular front (Left). The dashed line delineates the edge of the developing retina in A, B, and E. (Scale bars: Left, 500 um; Right, 100 um.) (C-E) Flat mounts showing the front of the developing vasculature in (C) control and (D and E) mutant retinas at P7 after intracardiac perfusion of DiI. The white square in E corresponds to the region shown in D. (Scale bars: C and D, 100 um; E, 500 um.) (F and G) Hypoxyprobe labeling of (F) control and (G) mutant retinas at P7. (Scale bar: 500 um.)

Copyright

This image is from Rattner A, Proc Natl Acad Sci U S A 2013 Oct 1;110(40):E3830-E3839. Copyright 2013 National Academy of Sciences, U.S.A. J:201133

Associated Alleles

Symbol	Name
Igs1^tm11(CAG-Bgeo,-Edn2)Nat	intergenic site 1; targeted mutation 11, Jeremy Nathans
Tg(Six3-cre)69Frty	transgene insertion 69, Yasuhide Furuta

Associated Genotypes

Allelic Composition	Genetic Background
Igs1^tm11(CAG-Bgeo,-Edn2)Nat/Igs1^+ Tg(Six3-cre)69Frty/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2