Phenotype Image Detail

Image

Caption

Interstitial tip cells in adult Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}/Igs1⁺ Tg(Pdgfra-cre)1Clc/0 (Z/Edn2;PDGFRalpha-Cre) retinas. (A) Flatmount adult control and mutant retinas immunostained for Tie2 and PECAM1 were imaged at the level of the OPL. The three mutant examples (mouse numbers 4035, 4730, and 4731) show capillaries terminating with blind endings decorated by multiple filopodia (arrowheads) and reduced levels of Tie2. The control mouse (4732) shows only the normal intraretinal capillary plexus. Column 4 shows the anti-PECAM1 images in grayscale, with intensities inverted for greater clarity. Many capillaries that enter or exit the focal plane appear truncated in these images, but these capillaries are readily distinguished from blind endings by examining adjacent focal planes. Additionally, normal adult capillary ECs express Tie2 and lack filopodia. (Scale bar: 50 um.) (B) Quantification of blind endings in adult control and mutant retinas. Each symbol represents one retina. Means and SDs are shown. The Ndp^tm2Nat/Ndp^tm2Nat Igs1^{tm2(CAG-Bgeo,-Ndp,-EGFP)Nat}/Igs1⁺ Tg(Slc1a3-cre/ERT)1Nat/0 retinas have a several fold lower OPL capillary density than wild-type control or mutant retinas. (C) The OPL capillary density in mutant retinas is elevated by ~15% compared with control retinas. Relative vascular density was determined by counting the number of intersections of GS-lectin-stained vessels with a set of six parallel lines, each of 500-um length, within an area of 500 um². Means, SDs, and P values are indicated.

Copyright

This image is from Rattner A, Proc Natl Acad Sci U S A 2013 Oct 1;110(40):E3830-E3839. Copyright 2013 National Academy of Sciences, U.S.A. J:201133

Associated Alleles

Symbol	Name
Igs1^tm11(CAG-Bgeo,-Edn2)Nat	intergenic site 1; targeted mutation 11, Jeremy Nathans
Tg(Pdgfra-cre)1Clc	transgene insertion 1, Constance L Cepko

Associated Genotypes

Allelic Composition	Genetic Background
Igs1^tm11(CAG-Bgeo,-Edn2)Nat/Igs1^+ Tg(Pdgfra-cre)1Clc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6