Phenotype Image Detail

Image

Caption

Spatially localized Edn2 expression results in local inhibition of retinal vascular development in Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}/Igs1⁺ Tg(Pax6-cre,GFP)2Pgr/0 (Z/Edn2;Pax6alpha-Cre) mice. (A, Upper) Sections of mutant retinas stained with GS-lectin after Vegfa in situ hybridization. (A, Lower) mutant retinas immunostained for Hypoxyprobe. (A, Left) Vascular architecture appears close to normal in the central retina, where Pax6alpha-Cre is not expressed; there is a low level of Vegfa expression in the INL and a low level of Hypoxyprobe accumulation. (A, Right) Hypertrophic blood vessels on the vitreal face of the retina with attenuated intraretinal projections are seen in the peripheral retina, where Cre-mediated recombination has activated Edn2 expression from the Z/Edn2 locus. This region has high expression of Vegfa in Muller glia and high levels of Hypoxyprobe. (Scale bar: 200 um.) (B) Flat mount of control and mutant retinas stained for Hypoxyprobe and GS-lectin. (Scale bar: 500 um.) (C) The front of the IPL vasculature in an adult mutant retina immunostained for Tie2 and PECAM1. Arrowheads point to several ECs at the vascular front that are decorated with filopodia and express low levels of Tie2. The retinal center is to the left, and the periphery is to the right. (Scale bar: 100 um.)

Copyright

This image is from Rattner A, Proc Natl Acad Sci U S A 2013 Oct 1;110(40):E3830-E3839. Copyright 2013 National Academy of Sciences, U.S.A. J:201133

Associated Alleles

Symbol	Name
Igs1^tm11(CAG-Bgeo,-Edn2)Nat	intergenic site 1; targeted mutation 11, Jeremy Nathans
Tg(Pax6-cre,GFP)2Pgr	transgene insertion 2, Peter Gruss

Associated Genotypes

Allelic Composition	Genetic Background
Igs1^tm11(CAG-Bgeo,-Edn2)Nat/Igs1^+ Tg(Pax6-cre,GFP)2Pgr/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6