Phenotype Image Detail

Image

Caption

Inhibition of vascular development and midgestational lethality in Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}/Igs1⁺ Edil3^{Tg(Sox2-cre)1Amc}/0 (Z/Edn2;Sox2-Cre) embryos. (A and B) Dorsal view of E8.5 control and mutant embryos, showing mild growth retardation in the mutant embryo. (C and D) Lateral view of E9.5 control and mutant embryos, showing severe growth retardation in the mutant embryo, with pooling of blood in the head and thorax and dilated cardiac chambers (arrowhead in D). (E and F) Transverse sections of Toluidine Blue-stained E9.5 control and mutant embryos. (E) The control embryo has distinct atrial and ventricular chambers and a closed neural tube. (F) The mutant embryo has greatly enlarged and partially distinct atrial and ventricular chambers and an open neural tube. (Scale bar: 200 um.) (G and H) Flat-mount PECAM1 staining (with an HRP-conjugated secondary antibody and purple/black reaction product) of E9.5 control and mutant embryos. (G) The control embryo shows a well-organized segmental vasculature. (H) The mutant embryo shows minimal vascular development. (I and J) Deletion of Ednra can rescue the midgestational growth retardation of mutant embryos, which is shown by the normal appearance of the E14.5 Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}/Igs1⁺ Edil3^{Tg(Sox2-cre)1Amc}/0 Ednra^tm2Ywa/Ednra^tm2Ywa (Z/Edn2; Sox2-Cre; Ednra^f/-) embryo.

Copyright

This image is from Rattner A, Proc Natl Acad Sci U S A 2013 Oct 1;110(40):E3830-E3839. Copyright 2013 National Academy of Sciences, U.S.A. J:201133

Associated Alleles

Symbol	Name
Edil3^Tg(Sox2-cre)1Amc	EGF-like repeats and discoidin I-like domains 3; transgene insertion 1, Andrew P McMahon
Igs1^tm11(CAG-Bgeo,-Edn2)Nat	intergenic site 1; targeted mutation 11, Jeremy Nathans

Associated Genotypes

Allelic Composition	Genetic Background
Igs1^tm11(CAG-Bgeo,-Edn2)Nat/Igs1^+ Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA