Phenotype Image Detail

Image

Caption

Comparison of filopodia density in wild-type vs. Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}/Igs1⁺ Tg(Six3-cre)69Frty/0 (Z/Edn2;Six3-Cre) retinal vasculature at P7. (A-H) Four representative images are shown for each genotype. Surface vasculature is visualized with GS-lectin staining, and filopodia are scored with red dots when they are at the growing vascular front and green dots when they are in the interior. We note that the higher density of endothelial cells in the mutant plexus hampers a rigorous comparison of the filopodial density between the two samples behind the vascular front, because the only filopodia that can be reliably scored are the filopodia that traverse a cell-free zone. (Scale bar: 100 um.)

Copyright

This image is from Rattner A, Proc Natl Acad Sci U S A 2013 Oct 1;110(40):E3830-E3839. Copyright 2013 National Academy of Sciences, U.S.A. J:201133

Associated Alleles

Symbol	Name
Igs1^tm11(CAG-Bgeo,-Edn2)Nat	intergenic site 1; targeted mutation 11, Jeremy Nathans
Tg(Six3-cre)69Frty	transgene insertion 69, Yasuhide Furuta

Associated Genotypes

Allelic Composition	Genetic Background
Igs1^tm11(CAG-Bgeo,-Edn2)Nat/Igs1^+ Tg(Six3-cre)69Frty/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2