Phenotype Image Detail

Image

Caption

Effect of mutations in Wfikkn2 (Gasp1) and Wfikkn1 (Gasp2) on skeletal muscle. (A) Reductions in muscle mass in Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl (Gasp1^-/-) and Wfikkn1^tm1.1Sjl/Wfikkn1^tm1.1Sjl (Gasp2^-/-) mice. Numbers represent percent increase or decrease in muscle mass relative to wild-type (WT). Muscles analyzed were pectoralis (red), triceps (gray), quadriceps (blue), and gastrocnemius (green). *P < 0.01 vs. WT;⁺P < 0.001 vs. WT. (B) Fiber type analysis of the gastrocnemius muscle. Note that muscles of Gasp1^-/- and Gasp2^-/- mice had an increased ratio of myosin heavy chain IIa (green) to myosin heavy chain IIb (red) positive fibers compared with WT mice, which was most evident in the ventrolateral portion of the muscle. The boxed region is shown at higher magnification next to the image of the entire gastrocnemius muscle. (C) Quantification of the ratio of IIa to IIb fibers. Analysis of 2,000 myofibers in the ventrolateral region of the gastrocnemius was carried out for three mice per group. Note the increase in the percentage of IIa fibers in Gasp1^-/- (P = 0.014) and Gasp2^-/- (P = 0.017) mice and the decrease in the percentage of IIb fibers in Gasp1^-/- (P = 0.010) and Gasp2^-/- (P = 0.018) mice compared with WT mice. Data are shown as mean SEM.

Copyright

This image is from Lee YS, Proc Natl Acad Sci U S A 2013 Sep 24;110(39):E3713-22. Copyright 2013 National Academy of Sciences, U.S.A. J:201148

Associated Alleles

Symbol	Name
Wfikkn1^tm1.1Sjl	WAP, FS, Ig, KU, and NTR-containing protein 1; targeted mutation 1.1, Sejin Lee
Wfikkn2^tm1.1Sjl	WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2; targeted mutation 1.1, Sejin Lee

Associated Genotypes

Allelic Composition	Genetic Background
Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl	involves: 129 * C57BL/6
Wfikkn1^tm1.1Sjl/Wfikkn1^tm1.1Sjl	involves: 129 * C57BL/6