Phenotype Image Detail

Image

Caption

Inner hair cell (IHC) stereocilia abnormalities in Tg(CAG-Diaph3)771Lesp/0 (771) and Tg(CAG-Diap3)924Lesp/0 (924) mice at 24 weeks of age. Scanning electron microscopy images of the organ of Corti were obtained from wild-type (WT) mice, line 771, and line 924. Scale bars in A-C represent 10 um; scale bars in D-F represent 1 um. (A) Stereocilia of WT outer hair cells (OHCs) and inner hair cells (IHCs) appeared distinct and organized. (B-C) In contrast, IHC stereocilia of line 771 (B) and line 924 (C) display abnormal fusion, occur in a single row, and are substantially reduced in numbers. In some regions, OHC hair bundles were splayed or flattened (B). At higher magnification, the fused appearance of the IHC stereocilia in line 771 (E) and line 924 (F) becomes more obvious in comparison to the WT hair bundle (D). Note the stereocilium marked with an arrow in (F) that appears to have three tips to a single base. Apical bulging of the IHC cell bodies is noted but may represent artifact.

Copyright

This image is from Schoen CJ, PLoS One 2013;8(2):e56520, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:193237

Associated Alleles

Symbol	Name
Tg(CAG-Diap3)924Lesp	transgene insertion 924, Marci Lesperance
Tg(CAG-Diaph3)771Lesp	transgene insertion 771, Marci Lesperance

Associated Genotypes

Allelic Composition	Genetic Background
Tg(CAG-Diaph3)771Lesp/0	FVB/NJ-Tg(CAG-Diaph3)771Lesp
Tg(CAG-Diap3)924Lesp/0	FVB/NJ-Tg(CAG-Diap3)924Lesp