Phenotype Image Detail

Image

Caption

Activated fibrogenic response in Glmp^{Gt(P084H04)Wrst}/Glmp^{Gt(P084H04)Wrst} (Ncu-g1^gt/gt) liver.(A, B) Immunofluorescence image shows CD68- and F4/80-stained Kupffer cells (KCs) (green). Liver sections were counter-stained with DAPI (blue). Infiltrating leukocytes can be seen in mutant liver section (white arrow). CD68-positive cells seem to congregate at the site of leukocyte infiltration. In mutant liver sections, F4/80-positive cells appear swollen and hypertrophic. Scale bars: 50 um. (G) This was supported by alpha-Sma staining in liver sections from wild-type (WT) and mutant mice. Note the positive labeling of alpha-Sma indicating activated hepatic stellate cells (HSCs) (arrows) in affected areas. Liver sections were counterstained with hematoxylin. Scale bar: 50 um. (H) Immunofluorescence imaging shows increased levels of myeloperoxidase at the protein level in mutant liver (green). Liver sections were counterstained with DAPI (blue). Note the granular staining pattern of myeloperoxidase, clustering of myeloperoxidase-positive cells and increased levels of invading polymorphonuclear leukocytes with small nuclei. Scale bar: 50 um

Copyright

This image is from Kong XY, Dis Model Mech 2014 Mar;7(3):351-62, and is displayed under the terms of the Creative Commons Attribution-Noncommerical-Share Alike 3.0 Unported License. J:208774

Associated Alleles

Symbol	Name
Glmp^Gt(P084H04)Wrst	glycosylated lysosomal membrane protein; gene trap P084H04, German Gene Trap Consortium

Associated Genotypes

Allelic Composition	Genetic Background
Glmp^Gt(P084H04)Wrst/Glmp^Gt(P084H04)Wrst	involves: 129S2/SvPas * C57BL/6