Phenotype Image Detail

Image

Caption

Proteoglycan and collagen II distributions and detection of Errfi1 (Mig-6) expression in the knee joints of Errfi1^tm3.1Gvw/Errfi1^tm3.1Gvw Tg(Col2a1-cre)1Asz/0 (Mig-6^flox/flox;Col2a1^Cre) and the control (Mig-6^flox/+;Col2a1^Cre) mice. (A) Safranin O staining was used to detect proteoglycan in the knee joints of the control and mutant mice at 3 and 15.3 months of age. The osteophytes (labeled "nod") in the mutant joints at both ages produced an abundance of proteoglycans. The thickness of the proteoglycan-positive articular cartilage (AC) in both the femur and tibia was significantly increased in the mutant joints especially in older mice, relative to those of control joints. The distribution of proteoglycans in the growth plates (GP) of both strains appeared similar. (B) IHC staining of collagen II in the articular cartilage and growth plate of 3-month-old control and mutant knee joints. (C) Detection of Errfi1 (Mig-6) expression in the control and mutant knee joints by IHC staining. The arrows indicate representative positively stained cells.

Copyright

This image is from Staal B, Proc Natl Acad Sci U S A 2014 Feb 18;111(7):2590-5. Copyright 2014 National Academy of Sciences, U.S.A. J:206807

Associated Alleles

Symbol	Name
Errfi1^tm3.1Gvw	ERBB receptor feedback inhibitor 1; targeted mutation 3.1, George F Vande Woude
Tg(Col2a1-cre)1Asz	transgene insertion 1, Attila Aszodi

Associated Genotypes

Allelic Composition	Genetic Background
Errfi1^tm3.1Gvw/Errfi1^tm3.1Gvw Tg(Col2a1-cre)1Asz/0	involves: 129S1/Sv * 129S4/SvJaeSor * C57BL/6 * CBA