Phenotype Image Detail

Image

Caption

Fluorescent images of sperm ejaculated from Semg1^tm1Kemi/Semg1^tm1Kemi Tg(CAG-RFP,Acr-EGFP)RBGS002Osb/0 or Semg1^tm2Kemi/Semg1^tm2Kemi Tg(CAG-RFP,Acr-EGFP)RBGS002Osb/0 (SVS2^-/-Tg^RBGS) to the female reproductive tract. Female reproductive tracts were isolated 3 hours after sperm ejaculation. Green: acrosome-intact sperm; red: sperm mitochondria; blue: nuclei stained with DAPI. Arrows, acrosome-reacted sperm without the green-colored head. Asterisks, eggs. Scale bars: 25 um.

Copyright

This image is from Kawano N, Proc Natl Acad Sci U S A 2014 Mar 18;111(11):4145-50. Copyright 2014 National Academy of Sciences, U.S.A. J:207389

Associated Alleles

Symbol	Name
Semg1^tm1Kemi	semenogelin 1; targeted mutation 1, Kenji Miyado
Semg1^tm2Kemi	semenogelin 1; targeted mutation 2, Kenji Miyado
Tg(CAG-RFP,Acr-EGFP)RBGS002Osb	transgene insertion RBGS002, Research Institute for Microbial Diseases, Osaka University

Associated Genotypes

Allelic Composition	Genetic Background
Semg1^tm1Kemi/Semg1^tm1Kemi Tg(CAG-RFP,Acr-EGFP)RBGS002Osb/0	involves: 129 * C57BL/6 * C57BL/6N * DBA/2
Semg1^tm2Kemi/Semg1^tm2Kemi Tg(CAG-RFP,Acr-EGFP)RBGS002Osb/0	involves: 129 * C57BL/6 * C57BL/6N * DBA/2