Phenotype Image Detail

Image

Caption

Histology and microCT analysis of Snx10^{tm1c(EUCOMM)Raba}/Snx10^{tm1c(EUCOMM)Raba} Ctsk^tm1(cre)Ska/Ctsk^tm1(cre)Ska (Snx10 OC KO) and Snx10^{tm1a(EUCOMM)Raba}/Snx10^{tm1a(EUCOMM)Raba} (Snx10 KD) mice. A) Surface images of the femur (top), longitudinal mid-plane sections (middle) and skull images (bottom) show that osteoclast-specific Snx10 deficiency (Snx10 OC KO) leads to severely osteopetrotic bone, but unlike the global deficiency it does not result in a lack of cortical bone. The white line in the longitudinal mid-plane sections (A, middle) indicates the location of the transverse microCT images were taken. The transverse images show that, in contrast to the Snx10 KD mouse, Snx10 OC KO mice have cortical bone. B) Snx10 OC KO mice are normo-calcemic, suggesting that osteoclast-specific Snx10 deficiency does not have a major impact in calcium homeostasis. C) (top panel) H&E staining of longitudinal sections of the proximal femur show abundant unresorbed cartilage in Snx10 OC KO and Snx10 KD mice, typical of osteopetrosis. The growth plate (GP) is thicker in Snx10 KD mice. C) (FEMUR) Von Kossa/van Gieson staining of femur transverse sections close to the growth plate (cut at the depth of the white line shown in the upper panels) show numerous trabeculae in both mutant tibiae, confirming the osteopetrotic phenotype. However, while the trabeculae of Snx10 KD mice are not fully mineralized as evidenced by the pink stained osteoid (right panels), the trabeculae of osteoclast-specific Snx10 KO mice are fully mineralized (bottom panels). Similar results were observed for vertebra (C. VERTEBRA panel) and bones of the skull base/floor (C. SKULL panel).

Copyright

This image is from Ye L, PLoS Genet 2015 Mar;11(3):e1005057, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:224711

Associated Alleles

Symbol	Name
Ctsk^tm1(cre)Ska	cathepsin K; targeted mutation 1, Shigeaki Kato
Snx10^tm1a(EUCOMM)Raba	sorting nexin 10; targeted mutation 1a, Ricardo A Battaglino
Snx10^tm1c(EUCOMM)Raba	sorting nexin 10; targeted mutation 1c, Ricardo A Battaglino

Associated Genotypes

Allelic Composition	Genetic Background
Ctsk^tm1(cre)Ska/Ctsk^+ Snx10^tm1c(EUCOMM)Raba/Snx10^tm1c(EUCOMM)Raba	involves: 129S4/SvJae * C57BL/6 * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj