Phenotype Image Detail

Image

Caption

Snx10^{tm1a(EUCOMM)Raba}/Snx10^{tm1a(EUCOMM)Raba} oseoclasts are unable to acidify, have impaired endosomal pathways, and have defective ruffled borders. Acridine orange indicates acidification by osteoclasts derived from wild-type (WT) splenocytes (A), whereas none is present in Snx10^{tm1a(EUCOMM)Raba}/Snx10^{tm1a(EUCOMM)Raba} (Snx10 KD) osteoclasts (B) (Nuclei labeled green). (C) WT osteoclasts can internalize fluorescent dextran (green) but mutant osteoclasts (D) cannot. E) TEM images of bone sections show a well-developed ruffled border in WT osteoclasts (black arrowheads). F) Mutant osteoclasts form a severely defective ruffled border. BM: bone matrix. (HV = 80.0 kV).

Copyright

This image is from Ye L, PLoS Genet 2015 Mar;11(3):e1005057, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:224711

Associated Alleles

Symbol	Name
Snx10^tm1a(EUCOMM)Raba	sorting nexin 10; targeted mutation 1a, Ricardo A Battaglino

Associated Genotypes

Allelic Composition	Genetic Background
Snx10^tm1a(EUCOMM)Raba/Snx10^tm1a(EUCOMM)Raba	involves: 129S4/SvJae * C57BL/6 * C57BL/6J