Phenotype Image Detail

Image

Caption

Histology and immunohistochemistry of decalcified long bones from Tg(Col1a1-tTA)139Niss/0 Tg(tetO-HTR4*D100A)7Niss/0 (Coll(2.3)+/Rs1+) and wild-type (WT) 9-week-old mice. (A) Low magnification H&E image of a mutant humerus cross section showing a region of bone marrow hematopoietic cells (bm), surrounded by regions of abnormal bone containing uniform stromal-type cells (sc). (Scale bar, 500 mm.) (B) High-magnification H&E image of a bone marrow-containing region from A. Note the presence of normal-appearing osteoblasts near the surface of the bone (black arrowhead), with nearby abnormal cells (yellow arrowhead) that are not associated with a bone spicule, similar to that seen in C. (C) High-magnification H&E image of an abnormal bone region from A showing the small, uniform stromal-type cells between the trabeculi. (D and E) FLAG immunohistochemistry on ethanol-fixed mutant humeral bone sections demonstrates cytoplasmic staining for Tg(tetO-HTR4*D100A)7Niss expression (brown). (F-I) Osteocalcin (OC) immunohistochemistry on formalin-fixed WT (F and G) and mutant (H and I) femoral sections, demonstrating that cytoplasmic and extra-cellular osteocalcin is present in the bone lesions, confirming the presence of matured osteoblasts. (J-M) Osterix (Osx) immunohistochemistry on formalin-fixed WT (J and K) and mutant (L and M) femoral sections, demonstrating significant nuclear staining (brown; yellow arrowheads) by cells throughout the mutant bony lesions but only in the trabecular bone at the epiphysis of WT bones. (Scale bars 20 mm.)

Copyright

This image is from Hsiao EC, Proc Natl Acad Sci U S A 2008 Jan 29;105(4):1209-14. Copyright 2008 National Academy of Sciences, U.S.A. J:131617

Associated Alleles

Symbol	Name
Tg(Col1a1-tTA)139Niss	transgene insertion 139, Robert Nissenson
Tg(tetO-HTR4*D100A)7Niss	transgene insertion 7, Robert A Nissenson

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Col1a1-tTA)139Niss/0 Tg(tetO-HTR4*D100A)7Niss/0	FVB/N-Tg(Col1a1-tTA)139Niss Tg(tetO-HTR4*D100A)7Niss