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Caption | Histology and immunohistochemistry of decalcified long bones from Tg(Col1a1-tTA)139Niss/0 Tg(tetO-HTR4*D100A)7Niss/0 (Coll(2.3)+/Rs1+) and wild-type (WT) 9-week-old mice. (A) Low magnification H&E image of a mutant humerus cross section showing a region of bone marrow hematopoietic cells (bm), surrounded by regions of abnormal bone containing uniform stromal-type cells (sc). (Scale bar, 500 mm.) (B) High-magnification H&E image of a bone marrow-containing region from A. Note the presence of normal-appearing osteoblasts near the surface of the bone (black arrowhead), with nearby abnormal cells (yellow arrowhead) that are not associated with a bone spicule, similar to that seen in C. (C) High-magnification H&E image of an abnormal bone region from A showing the small, uniform stromal-type cells between the trabeculi. (D and E) FLAG immunohistochemistry on ethanol-fixed mutant humeral bone sections demonstrates cytoplasmic staining for Tg(tetO-HTR4*D100A)7Niss expression (brown). (F-I) Osteocalcin (OC) immunohistochemistry on formalin-fixed WT (F and G) and mutant (H and I) femoral sections, demonstrating that cytoplasmic and extra-cellular osteocalcin is present in the bone lesions, confirming the presence of matured osteoblasts. (J-M) Osterix (Osx) immunohistochemistry on formalin-fixed WT (J and K) and mutant (L and M) femoral sections, demonstrating significant nuclear staining (brown; yellow arrowheads) by cells throughout the mutant bony lesions but only in the trabecular bone at the epiphysis of WT bones. (Scale bars 20 mm.) | ||||||
Copyright | This image is from Hsiao EC, Proc Natl Acad Sci U S A 2008 Jan 29;105(4):1209-14. Copyright 2008 National Academy of Sciences, U.S.A. J:131617 | ||||||
Associated Alleles |
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/02/2024 MGI 6.24 |
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