Phenotype Image Detail

Image

Caption

Golgi images of dendritic spines in the apical and basal dendrites of control (CTL), Tg(CAG-lacZ,-FUS,-EGFP)629Gyu/0 Meox2^tm1(cre)Sor/Meox2⁺ (CAG-FUS^WT) and Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu/0 Meox2^tm1(cre)Sor/Meox2⁺ (CAG-FUS^R521G) mice. Spine density was analyzed in a total of 30 neurons from cortical layers IV-V in P18 Tg(CAG-lacZ,-FUS,-EGFP)629Gyu/0 Meox2^tm1(cre)Sor/Meox2⁺ (CAG-FUS^WT) and Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu/0 Meox2^tm1(cre)Sor/Meox2⁺ (CAG-FUS^R521G) mice and their corresponding littermate controls (n = 3 mice per group). In Tg(CAG-lacZ,-FUS,-EGFP)629Gyu/0 Meox2^tm1(cre)Sor/Meox2⁺ (CAG-FUS^WT) mice the density of mature dendritic spines does not differ in apical and basal dendrites. However, in Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu/0 Meox2^tm1(cre)Sor/Meox2⁺ (CAG-FUS^R521G) mice the density of mature dendritic spines is reduced in the apical and secondary dendrites compared with littermate controls. (We have defined "mature" spines as being mushroom-shaped.) Arrowheads indicate immature spines.

Copyright

This image is from Sephton CF, Proc Natl Acad Sci U S A 2014 Nov 4;111(44):E4769-78. Copyright 2014 National Academy of Sciences, U.S.A. J:216672

Associated Alleles

Symbol	Name
Meox2^tm1(cre)Sor	mesenchyme homeobox 2; targeted mutation 1, Philippe Soriano
Tg(CAG-lacZ,-FUS,-EGFP)629Gyu	transgene insertion 629, Gang Yu
Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu	transgene insertion 682, Gang Yu

Associated Genotypes

Allelic Composition	Genetic Background
Meox2^tm1(cre)Sor/Meox2^+ Tg(CAG-lacZ,-FUS,-EGFP)629Gyu/0	involves: 129S4/SvJaeSor * C57BL/6
Meox2^tm1(cre)Sor/Meox2^+ Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu/0	involves: 129S4/SvJaeSor * C57BL/6