Phenotype Image Detail

Image

Caption

Bouquet release is significantly delayed in Lmna^tm1Rben/Lmna^tm1Rben (Lamin C2-/-) spermatocytes. (A) Representative wild-type (Lamin C2+/+) and knockout spermatocytes from testes of 10 to 14 day old mice labelled for SYCP3 and SUN1 with clusters of telomeres indicated by asterisks. Scale bars 5 um. (B) Z-projections of entire nuclei from wild-type and mutant littermates, labelled again for SYCP3 and SUN1, demonstrating the distinguishable difference between clustered and non-clustered telomere patterns; asterisks indicate telomere clusters. Scale bar 5 um. (C) Quantitative evaluation of spermatocytes showing clustered telomeres in wild-type and mutant littermates aged 10 to 14 dpp. Note the highly significant differences at ages 13 and 14 dpp. Values represent means (+/- s.d.) of data from three littermate pairs of wild-type and mutant animals each, separately analysed using Pearson's Chi² tests: d10 p=0.28 (wt, n=517; mutant, n=580); d11 p=0.15 (wt, n=677; mutant, n=703); d12 p=0.29 (wt, n=736; mutant, n=780); d13 p = 6.5 x10^-3 (wt, n=639; mutant, n=648); d14 p = 7.6 x10^-7 (wt, n=700; mutant, n=682). ***p<0.01 (D) Representative wild-type and mutant spermatocytes from day 14 aged mice showing comparable stages of synaptonemal complex assembly as indicated by SYCP1 labelling. Arrowheads denote homologous pairing defects in the absence of lamin C2; the asterisks indicate telomere clustering. Scale bar 5 um.

Copyright

This image is from Link J, PLoS Genet 2013;9(1):e1003261, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:223056

Associated Alleles

Symbol	Name
Lmna^tm1Rben	lamin A; targeted mutation 1, Ricardo Benavente

Associated Genotypes

Allelic Composition	Genetic Background
Lmna^tm1Rben/Lmna^tm1Rben	involves: 129S1/Sv * 129X1/SvJ