Phenotype Image Detail

Image

Caption

Cre-mediated recombination in Tsc1^tm1Djk/Tsc1^tm1Djk Gt(ROSA)26Sor^tm1Sho/Gt(ROSA)26Sor⁺ mice after neonatal delivery of AAV1-CBA-Cre vector (2x10¹⁰ g.c. per 2 ul into each ventricle). (A) Three months after ICV vector injection into Tsc1^tm1Djk/Tsc1^tm1Djk Gt(ROSA)26Sor^tm1Sho/Gt(ROSA)26Sor⁺ homozygous pups, frozen sections were stained with X-gal and counterstained with Nuclear Fast Red. Intense lacZ staining was visualized in clusters throughout the brain (coronal section, anterior to hippocampus region); note enlarged ventricles (V). Magnification = 2X. (B) The ventricular lining showed patches of lacZ staining and thickening. (C) Nodule-like structure, composed of cells in which Cre recombination has been induced as visualized by X-gal staining, were observed in the ventricles. (D) H&E staining showing nodule along ventricular lining. The regions in B, C & D are taken at a level close to the anterior amygdala. Magnification B, C & D = 10X.

Copyright

This image is from Prabhakar S, PLoS One 2013;8(5):e64224, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:221022

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1Sho	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Stuart Orkin
Tsc1^tm1Djk	TSC complex subunit 1; targeted mutation 1, David J Kwiatkowski

Associated Genotypes

Allelic Composition	Genetic Background
Tsc1^tm1Djk/Tsc1^tm1Djk Gt(ROSA)26Sor^tm1Sho/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * 129S4/SvJaeSor