Phenotype Image Detail

Image

Caption

Male Tysnd1^tm1.1Arte/Tysnd1^tm1.1Arte (Tysnd1-/-) mice are infertile. A. Semi-thin (8-10 micron) testes sections of 20 weeks old wild-type (WT) and homozygous mutant mice were stained with hematoxylin-eosin. Abnormal, round sperm heads are visible in the seminiferous tubules of mutant mice. B. Abnormal morphology of mutant sperms. Epididymal sperms of 15 weeks-old WT and mutant mice were stained with mitochondrial stains (MitoFluor Red) and nuclear staining (DAPI). Scale bar = 20 um. C. Percentage of sperms showing normal morphology in WT (n = 4), heterozygous (n = 4) and homozygous mutant (n = 5) mice. Each error bar represents the mean 6 SE. D. Anti-MN9 antibody immunostaining and Hoechst nuclear staining of epididymal sperms isolated from a 20 weeks old mutant mouse (red: acrosome and blue: nucleus). Arrow heads and arrows indicate abnormal round-headed sperms and normal sperms, respectively. Scale bar = 5 um. E. Acrosomes of a semi-thin testis section from a 10 weeks old homozygous mutant and a heterozygous control mouse were stained with PNA-FITC (green) and Hoechst nuclear stain (blue). F. EM image of a mutant caudal epididymal sperm. The round-headed sperm lacks the acrosome and shows an abnormal mitochondrial sheath (M) around the nucleus (N). Scale bar = 1 um. G. EM image showing normal spermatogenesis in heterozygous male mice. S7: step 7 round spermatid; S16: step 16 elongated spermatid. Acrosomes (A) are normally formed. Scale bar = 2 um. H. EM image of mutant elongated spermatid. S16: step 16 spermatid. In some spermatids the acrosome (*) is detached from the nucleus (N). Scale bar = 2 um.

Copyright

This image is from Mizuno Y, PLoS Genet 2013;9(2):e1003286, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:223054

Associated Alleles

Symbol	Name
Tysnd1^tm1.1Arte	trypsin domain containing 1; targeted mutation 1.1, TaconicArtemis

Associated Genotypes

Allelic Composition	Genetic Background
Tysnd1^tm1.1Arte/Tysnd1^tm1.1Arte	involves: C57BL/6J * C57BL/6N