Phenotype Image Detail

Image

Caption

HE-staining of dentate gyrus at each developmental stage for wild-type (+/+; WT) and Zfp521^tm1Nohk/Zfp521^tm1Nohk (delta/delta) mice. (A) HE-staining of dentate gyrus in five-week-old mice. Bars = 50 um. a, WT mouse; b, mutant mouse. Note that the density of neuronal cells was obviously lower and the neuronal cell layer was more obscure in the mutant mouse. (B) HE-staining of dentate gyrus in five-day-old mice. Bars = 100 um (a and b) and 50 um (c and d). a and c, WT mouse; b and d, mutant mouse. Note that the number of neuronal cells appear smaller in five-day-old mutant mouse than in WT mouse. (C) HE-staining of dentate gyrus and hippocampus in E19.5 embryos. Bars = 100 um. a, WT embryo; b, mutant embryo. Note that the dentate gyrus and hippocampus of the mutant embryo were poorly organized, and the number of cells in the neuronal cell layer was smaller in the mutant embryo than in the WT embryo. (D) Quantification of the number of cells in dentate gyrus. Hematoxylin positive cells were counted. Error bar indicates SD. n = 6. *,P<0.05, compared to WT mice. Note that the number of cells in dentate gyrus of both five-week-old (5w.o) and five-day-old (P5) mice were significantly decreased compared with WT littermates. Error bar indicates SD. n = 6.

Copyright

This image is from Ohkubo N, PLoS One 2014;9(3):e92848, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:214267

Associated Alleles

Symbol	Name
Zfp521^tm1Nohk	zinc finger protein 521; targeted mutation 1, Nobutaka Ohkubo

Associated Genotypes

Allelic Composition	Genetic Background
Zfp521^tm1Nohk/Zfp521^tm1Nohk	B6J.Cg-Zfp521^tm1Nohk