Phenotype Image Detail

Image

Caption

Rfx2^{Gt(IST10638H11)Tigm}/Rfx2⁺ (Rfx2^gt/+) and Rfx2^{Gt(IST10638H11)Tigm}/Rfx2^{Gt(IST10638H11)Tigm} (Rfx2^gt/gt) testis histology. Panels A, C, and E correspond to heterozygous. Panels B, D, and F correspond to the homozygous mutant. The boxed areas in panels A and B are enlarged in panels C-F. (a,b) Spermatozoa (s) are present in the lumen of seminiferous tubules from heterozygous mice but are absent in homozygous testis (*). The arrows point to the interstitial space containing Leydig cells, which appear enlarged in homozygous mice compared to heterozygous mice. The bar equals 100 um. (c,d) Elongating spermatids (El S'tids) are prominent in the seminiferous tubules of heterozygous testis and give rise to spermatozoa (arrowhead). In homozygous testis, elongating spermatids are replaced by symplasts of spermatids (Sym S'tids). Spermatocytes located basally (yellow arrowheads) are present in both heterozygous and homozygous testis sections. (e,f) Increased numbers of Leydig cells (Ley) are present in the interstitial space of homozygous testis compared with heterozygous testis.

Copyright

This image is from Shawlot W, Genesis 2015 Aug 6;():, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:227136

Associated Alleles

Symbol	Name
Rfx2^Gt(IST10638H11)Tigm	regulatory factor X, 2 (influences HLA class II expression); gene trap IST10638H11, Texas A&M Institute for Genomic Medicine

Associated Genotypes

Allelic Composition	Genetic Background
Rfx2^Gt(IST10638H11)Tigm/Rfx2^Gt(IST10638H11)Tigm	either: C57BL/6N-Rfx2^Gt(IST10638H11)Tigm or (involves: C57BL/6N * CD-1)