Phenotype Image Detail

Image

Caption

Abnormal mitochondrial sheath in Ddhd1^tm1.1Katn/Ddhd1^tm1.1Katn (-/-) spermatozoa. A, differential interference contrast images of spermatozoa derived from the testis (top row), caput epididymis (middle row), and cauda epididymis (bottom row) of wild-type (WT) and mutant mice. Insets show enlarged images of the boxed areas. Arrowheads indicate the segment between the mitochondrial sheath and the principal piece. Scale bar, 20 um. Quantitative data are shown at the bottom. At least 100 spermatozoa were counted for each sample. Data are means +/- S.D. (n=3). B, spermatozoa from WT and mutant mice were double stained with Hoechst 33342 (blue) and anti-alpha-tubulin antibody (green). Arrowheads indicate an abnormal microtubule structure. Scale bar, 20 um. Quantitative data are shown at the bottom. At least 100 spermatozoa were counted for each sample. Data are means +/- S.D. (n=3) C, spermatozoa from WT and mutant mice were double stained with Hoechst 33342 (blue) and MitoTracker Red CMXRos (red). Images were captured with an Olympus DP70 charge-coupled device camera attached to an Olympus IX70 microscope, and the length of the mitochondrial sheath was determined using ImageJ software. Quantitative data are shown in the bottom graph. Three mice were analyzed for each genotype. For each mouse, 20 spermatozoa were analyzed. Data are means +/- S.D.D, spermatozoa from the cauda epididymis of WT and mutant mice were analyzed by electron microscopy. Upper row scale bar, 2 um; lower row scale bar, 1 um. Error bars represent S.D.

Copyright

This image is from Baba T, J Biol Chem 2014 Apr 18;289(16):11497-511 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:212425

Associated Alleles

Symbol	Name
Ddhd1^tm1.1Katn	DDHD domain containing 1; targeted mutation 1.1, Katsuko Tani

Associated Genotypes

Allelic Composition	Genetic Background
Ddhd1^tm1.1Katn/Ddhd1^tm1.1Katn	involves: C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj