Phenotype Image Detail

Image

Caption

Premature differentiation within the prehypertrophic zone in the long bone growth plates in Flnb^tm1Vshn/Flnb^tm1Vshn (FlnB-/-) mice. (A) Col2a1 and Col10a1 double immunostaining on P1 null radius shows an abnormally increased region of overlapping expression for the proliferation and hypertrophic markers (open arrow). Quantitative analysis of the length of Col2a1⁺-Col10a1⁺ overlapping expression relative to the growth plate length. The Col2a1⁺-Col10a1⁺ overlapping region is increased in mutant mice at P1 and 2 week old age (17.7% vs 8.6%, 24.2% vs 19.1% in mutant and wild-type (WT), respectively). The ratio at P7 shows the similar, albeit not significant, trend of change. At P1, n samples = 6, for P7 and 2 week age, n samples = 3. (B) Pthr1 antibody immunostaining. Pthr1⁺ zones are thickened in the mutant radius at P1, P7 and 2 weeks. At 2 weeks, most of the chondrocytes become Pthr1⁺ in some mutant mice (open arrow). (C) Ihh antibody immunostaining. Ihh⁺ zones are thickened in the mutant radius at P1, P7 and 2 weeks. At 2 weeks, the Ihh⁺ zone covers both the prehypertrophic and hypertrophic zones in most of the mutant mice (open arrow). Scale bars = 200 um.

Copyright

This image is from Hu J, PLoS One 2014;9(2):e89352, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:213355

Associated Alleles

Symbol	Name
Flnb^tm1Vshn	filamin, beta; targeted mutation 1, Volney Sheen

Associated Genotypes

Allelic Composition	Genetic Background
Flnb^tm1Vshn/Flnb^tm1Vshn	involves: 129S6/SvEvTac * C57BL/6