Phenotype Image Detail

Image

Caption

Flnb^tm1Vshn/Flnb^tm1Vshn (FlnB^-/-) mouse chondrocytes display decreased proliferation at postnatal ages. (A) Immunostaining for Sox9 shows a decrease in the proportion of Sox9⁺ cells in growth plates of mutant mice at all detected ages. The data are quantified and graphically summarized to the right. (B) Immunofluorescent photomicrographs of E16.5 mutant and control radial bone growth plate after immunostaining for proliferation markers: BrdU (fluoroscein), a marker labeling cells entering into S-phase; Ki67 (fluoroscein), a marker for cells in the cell cycle; and phospho-histone H3 (PH3) (fluoroscein), an M-phase marker. Higher magnification images of the outlined boxes are to the right. There is an overall reduction in each of these markers within the proliferative zone after Flnb knockout at various ages. Statistical analyses (n>/=3 independent samples per experiment) shows that the percentage of cells positively labeled for Sox9, BrdU, Ki67, and PH3 in mutant cortex is decreased, respectively when compared to littermate controls. * = p<0.05, ** = p<0.01, *** = p<0.001 by t-test. Scale bar = 200 um for low magnification; 50 um for high magnification.

Copyright

This image is from Hu J, PLoS One 2014;9(2):e89352, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:213355

Associated Alleles

Symbol	Name
Flnb^tm1Vshn	filamin, beta; targeted mutation 1, Volney Sheen

Associated Genotypes

Allelic Composition	Genetic Background
Flnb^tm1Vshn/Flnb^tm1Vshn	involves: 129S6/SvEvTac * C57BL/6